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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1668| Biochemical characterization of recombinant L-asparaginase (AnsA) from Rhizobium etli, a member of an increasing Rhizobial-type family of L-asparaginases | |
| ANGELICA MORENO ENRIQUEZ Zahaed Evangelista Martínez EDITH GRACIELA GONZALEZ MONDRAGON ARTURO CALDERON FLORES Roberto Alejandro Arreguín Espinosa de los Monteros ERNESTO PEREZ RUEDA ALEJANDRO HUERTA SAQUERO | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| TYPE II ASPARAGINASE RHIZOBIUM ETLI BIOCHEMICAL CHARACTERIZATION | |
| We report the expression, purification, and characterization of L-asparaginase (AnsA) from Rhizobium etli. The enzyme was purified to homogeneity in a single-step procedure involving affinity chromatography, and the kinetic parameters K(m), V(max), and k(cat) for L-asparagine were determined. The enzymatic activity in the presence of a number of substrates and metal ions was investigated. The molecular mass of the enzyme was 47 kDa by SDS-PAGE. The enzyme showed a maximal activity at 50 degrees C, but the optimal temperature of activity was 37 degrees C. It also showed maximal and optimal activities at pH 9.0. The values of K(m), V(max), k(cat), and k(cat)/K(m) were 8.9 +/- 0.967 × 10⁻³ M, 128 +/- 2.8 U/mg protein, 106 +/- 2 s⁻¹, and 1.2 +/- 0.105 × 10⁴ M⁻¹s⁻¹, respectively. The L-asparaginase activity was reduced in the presence of Mn²⁺, Zn²⁺, Ca²⁺, and Mg²⁺ metal ions for about 52% to 31%. In addition, we found that NH₄⁺, L-Asp, D-Asn, and beta-aspartyl-hydroxamate in the reaction buffer reduced the activity of the enzyme, whereas L-Gln did not modify its enzymatic activity. This is the first report on the expression and characterization of the L-asparaginase (AnsA) from R. etli. Phylogenetic analysis of asparaginases reveals an increasing group of known sequences of the Rhizobialtype asparaginase II family. | |
| 05-01-2012 | |
| Artículo | |
| J. Microbiol. Biotechnol., 22(3), 292-300, 2012 | |
| Inglés | |
| Moreno-Enríquez, A., Evangelista-Martínez, Z., González-Mondragón, E. G., Calderón-Flores, A., Arreguín, R., Pérez-Rueda, E., & Huerta-Saquero, A. (2012). Biochemical characterization of recombinant L-asparaginase (AnsA) from Rhizobium etli, a member of an increasing rhizobial-type family of L-asparaginases. J. Microbiol. Biotechnol., 22(3), 292-300. | |
| BIOLOGÍA MOLECULAR DE PLANTAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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