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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1689| Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca | |
| LAURA YESENIA SOLÍS RAMOS Julio César Ortiz Pavón ANTONIO ANDRADE TORRES Romano Porras Murillo Arturo Brenes Angulo Enrique Castaño de la Serna | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.15517/RBT.V67I2SUPL.37208 | |
| COMMON BEAN RECALCITRANT SPECIES GENETIC TRANSFORMATION A. TUMEFACIENS GUSA ACTIVIT | |
| Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene. | |
| 2019 | |
| Artículo | |
| Revista de Biología Tropical, 67(2 SUPL), S83-S94 | |
| Inglés | |
| Solís-Ramos, L. Y., Ortiz-Pavón, J. C., Andrade-Torres, A., Porras-Murillo, R., Brenes-Angulo, A., & de la Serna, E. C. (2019). Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca. Revista de Biología Tropical, 67(2 SUPL), S83-S94 | |
| BIOLOGÍA MOLECULAR DE PLANTAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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