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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1822| Fibrillarin ribonuclease activity is dependent on the GAR domain and modulated by phospholipids | |
| Francisco Guillén-Chable ULISES RODRIGUEZ CORONA Alejandro Pereira-Santana ANDREA BAYONA HERNANDEZ Luis Carlos Rodríguez Zapata Cecilia Aquino Perez Lenka Šebestová Nicolas Vitale Pavel Hozak Enrique Castaño de la Serna | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.3390/cells9051143 | |
| NUCLEOLUS RIBONUCLEOLAR PARTICLE RRNA FIBRILLARIN PHOSPHOINOSITIDES VIRAL PROGRESSION | |
| Fibrillarin is a highly conserved nucleolar methyltransferase responsible for ribosomal RNA methylation across evolution from Archaea to humans. It has been reported that fibrillarin is involved in the methylation of histone H2A in nucleoli and other processes, including viral progression, cellular stress, nuclear shape, and cell cycle progression. We show that fibrillarin has an additional activity as a ribonuclease. The activity is affected by phosphoinositides and phosphatidic acid and insensitive to ribonuclease inhibitors. Furthermore, the presence of phosphatidic acid releases the fibrillarin-U3 snoRNA complex. We show that the ribonuclease activity localizes to the GAR (glycine/arginine-rich) domain conserved in a small group of RNA interacting proteins. The introduction of the GAR domain occurred in evolution in the transition from archaea to eukaryotic cells. The interaction of this domain with phospholipids may allow a phase separation of this protein in nucleoli. | |
| 2020 | |
| Artículo | |
| Cells, 9(5), 1143. https://doi.org/10.3390/cells9051143 | |
| Inglés | |
| Guillen-Chable, F., Corona, U. R., Pereira-Santana, A., Bayona, A., Rodríguez-Zapata, L. C., Aquino, C., Šebestová, L., Vitale, N., Hozak, P., & Castano, E. (2020). Fibrillarin Ribonuclease Activity is Dependent on the GAR Domain and Modulated by Phospholipids. Cells, 9(5), 1143. https://doi.org/10.3390/cells9051143 | |
| BIOLOGÍA MOLECULAR DE PLANTAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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| 2020_Guillen_Chable.pdf | 10.25 MB | Adobe PDF | Visualizar/Abrir |