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In vitro assessment of organic and residual fractions of nematicidal culture filtrates from thirteen tropical Trichoderma strains and metabolic profiles of most-active
FELICIA AMALIA MOO KOH
JAIRO CRISTOBAL ALEJO
MARIA FE ANDRES
Jesús M. Martín Serrano
Fernando Reyes
JOSE TUN
MARIA MARCELA GAMBOA ANGULO
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.3390/jof8010082
ETHYL ACETATE FRACTION
HRMS DEREPLICATION
TRICHODERMA GHANENSE
TRICHODERMA HARZIANUM
TRICHODERMA KONINGIOPSIS
TRICHODERMA VIRENS
The nematicidal properties of Trichoderma species have potential for developing safer biocontrol agents. In the present study, 13 native Trichoderma strains from T. citrinoviride, T. ghanense (2 strains), T. harzianum (4), T. koningiopsis, T. simmonsii, and T. virens (4) with nematicidal activity were selected and cultured in potato dextrose broth to obtain a culture filtrate (CF) for each. Each CF was partitioned with ethyl acetate to obtain organic (EA) and residual filtrate (RF) fractions, which were then tested on second-stage juveniles (J2s) of the nematodes Meloidogyne javanica and M. incognita in a microdilution assay. The most lethal strains were T. harzianum Th43-14, T. koningiopsis Th41-11, T. ghanense Th02-04, and T. virens Th32-09, which caused 51–100% mortality (%M) of J2s of both nematodes, mainly due to their RF fractions. Liquid chromatography–diode array detector-electrospray-high resolution mass spectrometry analysis of the most-active fractions revealed sesquiterpene and polyketide-like metabolites produced by the four active strains. These native Trichoderma strains have a high potential to develop safer natural products for the biocontrol of Meloidogyne species. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
2022
Artículo
Journal of Fungi, 8(1), 82, 2022.
Inglés
Moo-Koh, F.A.; Cristóbal-Alejo, J.; Andrés, M.F.; Martín, J.; Reyes, F.; Tun-Suárez, J.M.; Gamboa-Angulo, M. In Vitro Assessment of Organic and Residual Fractions of Nematicidal Culture Filtrates from Thirteen Tropical Trichoderma Strains and Metabolic Profiles of Most-Active. J. Fungi 2022, 8, 82.https://doi.org/10.3390/ jof8010082
BIOLOGÍA MOLECULAR
Versión publicada
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