Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1047
Direct colony polymerase chain reaction (PCR): An efficient technique to rapidly identify and distinguish Mycosphaerella fijiensis and Mycosphaerella musicola
ROBERTO CARLOS VAZQUEZ EUAN
ROSA GRIJALVA ARANGO
BARTOLOME HUMBERTO CHI MANZANERO
MIGUEL ALONSO TZEC SIMA
IGNACIO RODRIGO ISLAS FLORES
CECILIA MONICA RODRIGUEZ GARCIA
LETICIA PERAZA ECHEVERRIA
ANDREW CHRISTOPHER JAMES
GILBERTO MANZO SANCHEZ
BLONDY BEATRIZ CANTO CANCHE
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: 10.5897/AJB11.4044
SIGATOKA DISEASES
BANANA’S MYCOSPHAERELLA
MOLECULAR DIAGNOSIS
DIRECT COLONY PCR
Sigatoka disease is the most important threat for banana production worldwide. Many species of Mycosphaerella have been described from banana but, to date, the three species Mycosphaerella fijiensis, M. musicola and M. eumusae are the only species found to be pathogenic to banana. Reliable identification by classical methods requires expertise because these fungi produce similar symptoms and they are morphologically similar. For studies of ecology, genetic diversity and epidemiology their differentiation is crucial. Several laboratories have developed molecular protocols to differentiate these fungi. Currently, a number of primers targeting ribosomal sequences, actin, tubulin and histone 3 genes are available for diagnosis of the Sigatoka complex. In the present work, we report a direct colony-polymerase chain reaction (DC-PCR) approach to rapidly distinguish M. fijiensis and M. musicola strains in multiplex PCR reactions. This is the most economical and the fastest procedure reported so far for diagnosis of these two Mycosphaerella species, which are distributed in banana-growing regions in the world; the DC-PCR technique was also found to be amenable for the identification of mating type of M. fijiensis isolates. This DC-PCR may also be applicable to prepare DNA templates for basic PCR-based analyses in other fungi.
2012
Artículo
African Journal of Biotechnology, 11(33), 8172-8180, 2012
Inglés
Vázquez-Euán, R., Grijalva-Arango, R., Chi-Manzanero, B., Tzec-Simá, M., Islas-Flores, I., Rodríguez-García, C., ... & Canto-Canché, B. (2012). Direct colony polymerase chain reaction (PCR): An efficient technique to rapidly identify and distinguish Mycosphaerella fijiensis and Mycosphaerella musicola. African Journal of Biotechnology, 11(33), 8172-8180.
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

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