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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1048| Cloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis | |
| YENY LIZZET COUOH UICAB IGNACIO RODRIGO ISLAS FLORES NUVIA EUGENIA KANTUN MORENO MIGUEL ALONSO TZEC SIMA SANTY PERAZA ECHEVERRIA LIGIA GUADALUPE BRITO ARGAEZ LETICIA PERAZA ECHEVERRIA ROSA GRIJALVA ARANGO ANDREW CHRISTOPHER JAMES CECILIA MONICA RODRIGUEZ GARCIA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.5897/AJB11.2966 | |
| ABC TRANSPORTERS VIRULENCE FACTORS MGATR4 ORTHOLOG MYCOSPHAERELLA FIJIENSIS BLACK SIGATOKA MUSA SP. | |
| ABC transporters are membrane proteins that use the energy released from the hydrolysis of ATP to drive the transport of compounds across biological membranes. In some plants, pathogenic fungi ABC transporters play a role as virulence factors by mediating the export of plant defense compounds or fungal virulence factors. Mycosphaerella fijiensis, the causal agent of black Sigatoka disease in banana, is the main constraint for the banana industry worldwide. So far, little is known about molecular mechanism that it uses to infect the host. In this study, degenerated primers designed from fungal ABC transporters known to be involved in virulence were used to isolate homologs from M. fijiensis.Here, we reported the full cloning of MfAtr4 a putative ortholog of MgAtr4, an ABC transporter of the related Mycosphaerella graminicola with a function in virulence. Similarities and differences with its presumed ortholog MgAtr4 are described, and the putative function of MfAtr4 are discussed. Analysis of MfAtr4 gene expression in field banana samples exhibiting visible symptoms of black Sigatoka disease indicated a higher expression of MfAtr4 during the first symptomatic stages in comparison to the late necrotrophic phases, suggesting a role for MfAtr4 in the early stages of pathogenic development of M. fijiensis. | |
| 2012 | |
| Artículo | |
| African Journal of Biotechnology, 11(1), 54-79, 2012 | |
| Inglés | |
| Couoh-Uicab, Y., Islas-Flores, L., Kantún-Moreno, N., Zwiers, L. H., Tzec-Simá, M., Peraza-Echeverría, S., ... & Rodríguez-García, C. (2012). Cloning, in silico structural characterization and expression analysis of MfAtr4, an ABC transporter from the banana pathogen Mycosphaerella fijiensis. African Journal of Biotechnology, 11(1), 54-79. | |
| BIOLOGÍA Y QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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