Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1071
Identifying a carotenoid cleavage dioxygenase 4a gene and its efficient agrobacterium-mediated genetic transformationin Bixa orellana L.
MOHAN SANKARI
HRIDYA HEMACHANDRAN
AMIRTHA ANANTHARAMAN
Subramanian Babu
RENATA LOURDES BARBARA RIVERA MADRID
George Priya Doss C
DEVANAND P. FULZELE
RAMAMOORTHY SIVA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: 10.1007/s12010-016-2025-8
AGRO-INFECTION
APOCAROTENOIDS
BIXA ORELLANA
CAROTENOID CLEAVAGE DIOXYGENASE
CCD4A
RT-PCR
Carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavage oxygenases (CCOs). The apocarotenoids are economically important as it is known to have therapeutic as well as industrial applications. For instance, bixin from Bixa orellana and crocin from Crocus sativus are commercially used as a food colourant and cosmetics since prehistoric time. In our present study, CCD4a gene has been identified and isolated from leaves of B. orellana for the first time and named as BoCCD4a; phylogenetic analysis was carried out using CLUSTAL W. From sequence analysis, BoCCD4a contains two exons and one intron, which was compared with the selected AtCCD4, RdCCD4, GmCCD4 and CmCCD4a gene. Further, the BoCCD4a gene was cloned into pCAMBIA 1301, transformed into Agrobacterium tumefaciens EHA105 strain and subsequently transferred into hypocotyledons and callus of B. orellana by agro-infection. Selection of stable transformation was screened on the basis of PCR detection by using GUS and hptII specific primer, which was followed by histochemical characterization. The percent transient GUS expression in hypocotyledons and callus was 84.4 and 80 %, respectively. The expression of BoCCD4a gene in B. orellana was confirmed through RT-PCR analysis. From our results, the sequence analysis of BoCCD4a gene of B. orellana was closely related to the CsCCD4 gene of C. sativus, which suggests this gene may have a role in various processes such as fragrance, insect attractant and pollination.
2016
Artículo
Applied biochemistry and biotechnology, 179(5), 697-714, 2016
Inglés
Sankari, M., Hemachandran, H., Anantharaman, A., Babu, S., Madrid, R. R., Fulzele, D. P., & Siva, R. (2016). Identifying a Carotenoid Cleavage Dioxygenase 4a Gene and Its Efficient Agrobacterium-Mediated Genetic Transformation in Bixa orellana L. Applied biochemistry and biotechnology, 179(5), 697-714.
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

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