Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1096
Laccase gene expression and vinasse biodegradation by Trametes hirsuta strain Bm-2
RAUL TAPIA TUSSELL
DAISY DE LA CARIDAD PEREZ BRITO
CLAUDIA GUADALUPE TORRES CALZADA
ALBERTO CORTES VELAZQUEZ
LILIANA MARIA ALZATE GAVIRIA
RUBI DEL ROSARIO CHABLE VILLACIS
SARA ELENA SOLIS PEREIRA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
doi:10.3390/molecules200815147
TRAMETES HIRSUTA
LACASSES
GENE EXPRESSION
BIODEGRADATION
VINASSE
Vinasse is the dark-colored wastewater that is generated by bioethanol distilleries from feedstock molasses. The vinasse that is generated from molasses contains high amounts of pollutants, including phenolic compounds and melanoindin. The goal of this work was to study the expression of laccase genes in the Trametes hirsuta strain Bm-2, isolated in Yucatan, Mexico, in the presence of phenolic compounds, as well as its effectiveness in removing colorants from vinasse. In the presence of all phenolic compounds tested (guaiacol, ferulic acid, and vanillic acid), increased levels of laccase-encoding mRNA were observed. Transcript levels in the presence of guaiacol were 40 times higher than those in the control. The lcc1 and lcc2 genes of T. hirsuta were differentially expressed; guaiacol and vanillin induced the expression of both genes, whereas ferulic acid only induced the expression of lcc2. The discoloration of vinasse was concomitant with the increase in laccase activity. The highest value of enzyme activity (2543.7 U/mL) was obtained in 10% (v/v) vinasse, which corresponded to a 69.2% increase in discoloration. This study demonstrates the potential of the Bm-2 strain of T. hirsuta for the biodegradation of vinasse.
01-08-2015
Artículo
Molecules. 20(8), 15147-15157, 2015
Inglés
Tapia-Tussell, R., Pérez-Brito, D., Torres-Calzada, C., Cortés-Velázquez, A., Alzate-Gaviria, L., Chablé-Villacís, R., & Solís-Pereira, S. (2015). Laccase Gene Expression and Vinasse Biodegradation by Trametes hirsuta Strain Bm-2. Molecules, 20(8), 15147-15157.
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

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