Por favor, use este identificador para citar o enlazar este ítem:
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1097| Single nucleotide polymorphisms in partial sequences of the gene encoding the large sub-units of ADP-glucose pyrophosphorylase within a representative collection of 10 Musa genotypes | |
| MUHILAN MAHENDHIRAN JORGE HUMBERTO RAMIREZ PRADO ROSA MARIA ESCOBEDO GRACIA MEDRANO BLONDY BEATRIZ CANTO CANCHE MIGUEL ALONSO TZEC SIMA ROSA GRIJALVA ARANGO ANDREW CHRISTOPHER JAMES KAY | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.1016/j.ejbt.2014.04.004 | |
| HAPLOTYPES STARCH METABOLISM ENZYME VARIANTS | |
| ADP-glucose pyrophosphorylase (AGPase) is a rate-limiting enzyme catalyzing the first step in the 17 Q3 starch biosynthesis pathway in higher plants. To date, there are no reported variants or isoforms of the AGPase 18 enzyme in bananas (Musa spp. family Musaceae) as is the case of other plants. In this study, genomic DNA 19 sequences homologous to the gene encodingone of the large subunits of the enzyme were amplified from 10 20 accessions of the genusMusa, including representatives ofwild ancestors (AA and BB genomes), dessert bananas 21 (AA, AAA, AB and AAB genomes), plantains (AAB genome) and cooking bananas (ABB and AAA genomes), and 22 studied in order to find SNP based variations in Musa accessions. 23 Results: In the 810-base pair amplicons of the AGPase large sub-unit (LSU) gene analyzed in tenMusa accessions, 24 a total of 36 single nucleotide polymorphisms (SNPs) and insertions/deletions (indels)were found. Thephyloge- 25 netic analysis revealed fifteen distinct haplotypes, which were grouped into four variants. Deep examination of 26 SNPs in the 2nd exon in the LSU of AGPase showed that at seven locations, five SNPs altered their amino acid 27 sequence. 28 Conclusions: This work reveals the possible number of AGPase enzyme isoforms and their molecular levels in 29 banana. Molecular markers could be designed from SNPs present in these banana accessions. This information 30 could be useful for the development of SNP-based molecular markers for Musa germplasm, and alteration of 31 the allosteric properties of AGPase to increase the starch content and manipulate the starch quality of banana 32 fruits. | |
| 2014 | |
| Artículo | |
| Electronic Journal of Biotechnology, 17(3), 137-147, 2014 | |
| Inglés | |
| Mahendhiran, M., Ramirez-Prado, J. H., Medrano, R. M. E. G., Canto-Canché, B., Tzec-Simá, M., Grijalva-Arango, R., & James-Kay, A. (2014). Single nucleotide polymorphisms in partial sequences of the gene encoding the large sub-units of ADP-glucose pyrophosphorylase within a representative collection of 10 Musa genotypes. Electronic Journal of Biotechnology, 17(3), 137-147. | |
| BIOLOGÍA Y QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
Cargar archivos:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| 2014_AI_id31411_JorgeRamirez_BlondyCanto.pdf | 2.56 MB | Adobe PDF | Visualizar/Abrir |