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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1106| Endogenous GUS-like activity in Capsicum chinense Jacq. | |
| LAURA YESENIA SOLÍS RAMOS TOMAS AUGUSTO GONZALEZ ESTRADA ANTONIO ANDRADE TORRES GREGORIO DEL CARMEN GODOY HERNANDEZ ENRIQUE CASTAÑO DE LA SERNA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.2225/vol13-issue4-fulltext-3 | |
| ß-GLUCURONIDASE AGROBACTERIUM TUMEFACIENS CAPSICUM CHINENSE GENE UIDA | |
| The gene uidA, codes for β-glucuronidase, which is one of the reporters more frequently utilized in transgenic plants. However, this can only be use if the selected organism does not present endogenous GUS-like activity. In tissues of C. chinense we found a GUS-like activity showing different levels of intensity. Histochemical screening showed that endogenous GUS-like activitydecreased, or reduced significantly, in almost all tissues with exception of stament, when phosphate buffer was adjusted to pH 8. Subsequently, C. chinense zygotic embryo explants were transient transformed with Agrobacterium tumefaciens LBA4404 (pCAMBIA2301) and plantlets regenerated were histochemically stained in phosphate buffer pH 8. Observations of incubated tissues of C. chinense regenerants showed blue staining, suggesting expression of uidA. Incubated tissues of non-transformed regenerants did not show blue staining in phosphate buffer pH 8. The results show that for transformation experiments of C. chinense with uidA gene, pH 8 is recommended for histochemical staining. | |
| 2010 | |
| Artículo | |
| Electronic Journal of Biotechnology, 13(4), 2-3, 2010 | |
| Inglés | |
| Solís-Ramos, L. Y., González-Estrada, T., Andrade-Torres, A., Godoy-Hernández, G., & Castaño de la Serna, E. (2010). Endogenous GUS-like activity in Capsicum chinense Jacq. Electronic Journal of Biotechnology, 13(4), 2-3. | |
| BIOLOGÍA Y QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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