Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1215
Protein extraction for Cocos nucifera with and without lethal yellowing phytoplasma: gel-based proteomics
CELSO REYES MARTINEZ
DANAE MORALES ANGELES
MARIA DEL SOCORRO NARVAEZ CAB
JUAN ALBERTO OSUNA CASTRO
DANIEL ZIZUMBO VILLARREAL
CARLOS MARIANO OROPEZA SALIN
ELPIDIO PEÑA BELTRAN
Acceso Abierto
Atribución-NoComercial-SinDerivadas
COCOS NUCIFERA
PHYTOPLASMA
PHENOLS
PROTEOMICS
ECOTYPES
GEL ELECTROPHORESIS
PLANT EXTRACTS
METHANOL
PIGMENTS
Two-dimensional gel electrophoresis (2-DE) is one of the two methodologies to separate and identify gene expression at the proteome level. However, the separation of proteins from plant tissue extract is often complicate, because it is necessary to modify the protocols depending upon the type of tissue and upon the presence of different interfering compounds. In this work it is established a routine procedure for the application of proteomic analysis to study interaction between palm coconut and lethal yellowing (LY) phytoplasma. The characteristics of protocol include: (i) cleanup of pigments and contaminants of the trunk palm coconut tissue, using a solution of acetone with TCA and 2-mercaptoethanol. (ii) Phenol extraction of proteins in presence of methanol with ammonium acetate. 2-DE analysis revealed differences in palm coconut with and without LY agent and similitude between palm ecotypes.
2007
Artículo
Bulletin of Insectology, 60(2), 231-232, 2007.
Inglés
Reyes-Martinez, C., Morales-Angeles, D., Narvaez-Cab, M., Osuna-Castro, J. A., Zizumbo-Villarreal, D., Oropeza-Salin, C. M., & Pena-Beltran, E. (2007). Protein extraction for Cocos nucifera with and without lethal yellowing phytoplasma: gel-based proteomics. Bulletin of Insectology, 60(2), 231-232.
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

Upload archives


File SizeFormat 
2007_Reyes_Celso.pdf276.27 kBAdobe PDFView/Open