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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/145| In Silico Cloning and Characterization of the Glycerol-3-Phosphate Dehydrogenase (GPDH) Gene Family in the Green MicroalgaChlamydomonas reinhardtii | |
| SANTY PERAZA ECHEVERRIA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Glycerol-3-phosphate dehydrogenase (GPDH) catalyzes the conversion of dihydroxyacetone phosphate (DHAP) and NADH to glycerol-3-phosphate (G3P) and NAD ? . G3P is important as a precursor for glycerol and glycerolipid synthesis in microalgae. A GPDH enzyme has been previously purified from the green microalgaChlamydomonas reinhardtii, however, no genes coding for GPDH have been characterized before. In this study, we report the in silico characterization of three putativeGPDH genes from C. reinhardtii: CrGPDH1, CrGPDH2, and CrGPDH3. These sequences showed a significant similarity to characterized GPDHgenes from the microalgae Dunaliella salinaandDunaliella viridis. The prediction of the three-dimensional structure of the proteins showed the characteristic fold topology of GPDH enzymes. Furthermore, the phylogenetic analysis showed that the three CrGPDHs share the same clade with characterized GPDHs fromDunaliellasuggesting a common evolutionary origin and a similar catalytic function. In addition, the Ka /Ks ratios of these sequences suggested that they are under purifying selection. Moreover, the expression analysis showed a constitutive expression of CrGPDH1, while CrGPDH2and CrGPDH3were induced in response to osmotic stress, suggesting a possible role for these two sequences in the synthesis of glycerol as a compatible solute in osmoregulation, and perhaps also in lipid synthesis in C. reinhardtii. This study has provided a foundation for further biochemical and genetic studies of the GPDHfamily in this model microalga, and also opportunities to assess the potential of these genes to enhance the synthesis of TAGs for biodiesel production. | |
| 23-02-2012 | |
| Artículo | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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