Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1519
5-azacytidine: A promoter of epigenetic changes in the quest to improve plant somatic embryogenesis
PEDRO MANUEL OSORIO MONTALVO
Luis Alfonso Sáenz Carbonell
Clelia de la Peña Seaman
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.3390/ijms19103182
SOMATIC EMBRYOGENESIS
DNA METHYLATION
5-AZACYTIDINE
EPIGENETICS
HYPOMETHYLATION
PLANT TISSUE CULTURE
Somatic embryogenesis (SE) is a widely studied process due to its biotechnological potential to generate large quantities of plants in short time frames and from different sources of explants. The success of SE depends on many factors, such as the nature of the explant, the microenvironment generated by in vitro culture conditions, and the regulation of gene expression, among others. Epigenetics has recently been identified as an important factor influencing SE outcome. DNA methylation is one of the most studied epigenetic mechanisms due to its essential role in gene expression, and its participation in SE is crucial. DNA methylation levels can be modified through the use of drugs such as 5-Azacytidine (5-AzaC), an inhibitor of DNA methylation, which has been used during SE protocols. The balance between hypomethylation and hypermethylation seems to be the key to SE success. Here, we discuss the most prominent recent research on the role of 5-AzaC in the regulation of DNA methylation, highlighting its importance during the SE process. Also, the molecular implications that this inhibitor might have for the increase or decrease in the embryogenic potential of various explants are reviewed.
2018
Artículo
International journal of molecular sciences, 19(10), 3182, 2018.
Inglés
Osorio-Montalvo, P., Sáenz-Carbonell, L., & De-la-Peña, C. (2018). 5-Azacytidine: A Promoter of Epigenetic Changes in the Quest to Improve Plant Somatic Embryogenesis. International journal of molecular sciences, 19(10), 3182.
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

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