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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1540| Molecular cloning and functional analysis of a Na + -insensitive K + transporter of Capsicum chinense Jacq | |
| NANCY RUIZ LAU Emanuel Bojorquez Quintal BEGOÑA BENITO CASADO Ileana de la Caridad Echevarría Machado Lucila Aurelia Sánchez Cach María de Fátima Medina Lara Manuel Martínez Estévez | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| 10.3389/fpls.2016.01980 | |
| CAPSICUM CHINENSE HAK-TYPE TRANSPORTER K+-STARVATION POTASSIUM ROOTS SODIUM | |
| High-affinity K+ (HAK) transporters are encoded by a large family of genes and are ubiquitous in the plant kingdom. These HAK-type transporters participate in low- and high-affinity potassium (K+) uptake and are crucial for the maintenance of K+ homeostasis under hostile conditions. In this study, the full-length cDNA of CcHAK1 gene was isolated from roots of the habanero pepper (Capsicum chinense). CcHAK1 expression was positively regulated by K+ starvation in roots and was not inhibited in the presence of NaCl. Phylogenetic analysis placed the CcHAK1 transporter in group I of the HAK K+ transporters, showing that it is closely related to Capsicum annuum CaHAK1 and Solanum lycopersicum LeHAK5. Characterization of the protein in a yeast mutant deficient in high-affinity K+ uptake (WΔ3) suggested that CcHAK1 function is associated with high-affinity K+ uptake, with Km and Vmax for Rb of 50 μM and 0.52 nmol mg−1 min−1, respectively. K+ uptake in yeast expressing the CcHAK1 transporter was inhibited by millimolar concentrations of the cations ammonium (NH+4) and cesium (Cs+) but not by sodium (Na+). The results presented in this study suggest that the CcHAK1 transporter may contribute to the maintenance of K+ homeostasis in root cells in C. chinense plants undergoing K+-deficiency and salt stress. | |
| 2019 | |
| Artículo | |
| Frontiers in plant science, 7, 1980. | |
| Inglés | |
| Ruiz-Lau, N., Bojórquez-Quintal, E., Benito, B., Echevarría-Machado, I., Sánchez-Cach, L. A., Medina-Lara, M. D. F., & Martínez-Estévez, M. (2016). Molecular cloning and functional analysis of a Na+-insensitive K+ transporter of Capsicum chinense Jacq. Frontiers in plant science, 7, 1980. | |
| BIOLOGÍA Y QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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