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Natur ally occur r ing fat ty acid methyl ester s and ethyl ester s in the gr een micr oalga Chlamydomonas reinhardtii
LUIS FELIPE BARAHONA PEREZ
Acceso Abierto
Atribución-NoComercial-SinDerivadas
Biodiesel can be defined as fatty acid methyl esters (F AMEs) or fatty acid ethyl esters (FAEE) obtained from the transesterification of triacylglycerides (T AGs) from vegetable oils and animal fats; however , oleaginous microalgae are emerging as potential substitutes of these feedstocks for biodiesel production. The use of the green microalga Chlamy domona s reinha rdtii P . A. Dang as a model system for lipid metabolism studies offers the advantage that extensive physiological and genomic data are available. The presence of naturally occurring F AMEs has been reported previously in plants and microalgae. In this study , oil extracts of C. reinha rdtii batch cultures at the end of the growth phase were analyzed before and after the transesterification reaction to investigate the presence of naturally occurring F AMEs in this microalga. As a result, the presence of these compounds was observed in hexane oil extracts of C. reinha rdtii before transesterification. Five FAMEs were identified by gas chromatography-mass spectrometry (GC-MS) before and after transesterification, while one additional F AME appeared only after transesterification. Additionally , three F AEEs were also identified before and after the transesterification reaction. Therefore, naturally occurring F AMEs and F AEEs are reported in C. reinha rdtii. These results will pave the way for further studies on the biosynthesis of these compounds in green microalgae, and their potential use as biofuels.
01-02-2012
Artículo
INGENIERÍA Y TECNOLOGÍA
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