Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1610
In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease
IVAN ISIDRO CORDOVA LARA
Phil Jones
Nigel A. Harrison
Carlos Mariano Oropeza Salín
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.1046/j.1364-3703.2003.00152.x
BACTERIA (MICROORGANISMS)
COCOS NUCIFERA
PHYTOPLASMA
PROKARYOTA
DNA of the lethal yellowing (LY) phytoplasma was detected in 13 of 72 embryos from fruits of four diseased Atlantic tall coconut palms by polymerase chain reaction (PCR) assays employing phytoplasma universal rRNA primer pair P1/P7, nested LY group‐specific rRNA primer pair 503f/LY16Sr or LY phytoplasma‐specific nonribosomal primer pair LYF1/R1. Phytoplasma distribution in sectioned tissues from six PCR positive embryos was determined by in situ PCR and digoxigenin‐11‐deoxy‐UTP (Dig) labelling of amplification products. Dig‐labeled DNA products detected by colourimetric assay were clearly evident on sections from the same three embryos investigated in detail by in situ PCRs employing primer pairs P1/P7 or LYF1/R1. Deposition of blue–green stain on sections as a result of each assay was restricted to areas of the embryos corresponding to the plumule and cells ensheathing it. By comparison, similarly treated embryo sections derived from fruits of a symptomless Atlantic tall coconut palm were consistently devoid of any stain. Presence of phytoplasma DNA in embryo tissues suggests the possible potential for seed transmission which remains to be demonstrated.
2003
Artículo
Molecular Plant Pathology, 4(2), 99-108, 2003.
Inglés
Cordova, I., Jones, P., Harrison, N. A., & Oropeza, C. (2003). In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease. Molecular Plant Pathology, 4(2), 99-108.
CITOGENÉTICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

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