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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1801| Auxin perception in Agave is dependent on the species’ Auxin Response Factors | |
| VICTOR JESUS CANCINO GARCIA Jorge Humberto Ramírez Prado Clelia de la Peña Seaman | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1038/s41598-020-60865-y | |
| AUXIN AGAVE | |
| Auxins are one of the most important and studied phytohormones in nature. Auxin signaling and perception take place in the cytosol, where the auxin is sensed. Then, in the nucleus, the auxin response factors (ARF) promote the expression of early-response genes. It is well known that not all plants respond to the same amount and type of auxins and that the response can be very different even among plants of the same species, as we present here. Here we investigate the behavior of ARF in response to various auxins in Agave angustifolia Haw., A. fourcroydes Lem. and A. tequilana Weber var. Azul. By screening the available database of A. tequilana genes, we have identified 32 ARF genes with high sequence identity in the conserved domains, grouped into three main clades. A phylogenetic tree was inferred from alignments of the 32 Agave ARF protein sequences and the evolutionary relationship with other species was analyzed. AteqARF 4, 15, 21, and 29 were selected as a representative diverse sample coming from each of the different subclades that comprise the two main clades of the inferred phylogenetic reconstruction. These ARFs showed differential species-specific expression patterns in the presence of indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Interestingly, A. angustifolia showed different phenotypes in the presence and absence of auxins. In the absence of auxin, A. angustifolia produces roots, while shoots are developed in the presence of IAA. However, in the presence of 2,4-D, the plant meristem converts into callus. According to our results, it is likely that AteqARF15 participates in this outcome. | |
| 2020 | |
| Artículo | |
| Scientific reports, 10(1), 1-11, 2020. | |
| Inglés | |
| Cancino-García, V. J., Ramírez-Prado, J. H., & De-la-Peña, C. (2020). Auxin perception in Agave is dependent on the species’ Auxin Response Factors. Scientific reports, 10(1), 1-11. | |
| BIOLOGÍA MOLECULAR DE PLANTAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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| 2020_Cancino.pdf | 4.72 MB | Adobe PDF | Visualizar/Abrir |