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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2111| Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates | |
| Peter Hoboth Ondrej Sebesta Martin Sztacho Enrique Castaño de la Serna Pavel Hozak | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1016/j.mex.2021.101372 | |
| IMMUNOFLUORESCENCE WIDE-FIELD MICROSCOPY SUPER-RESOLUTION MICROSCOPY IMAGEJ NEAREST NEIGHBOR DISTANCE CELL NUCLEUS NUCLEAR ARCHITECTURE | |
| Single molecule localization microscopy (SMLM) provided an unprecedented insight into the sub-nuclear organization of proteins and nucleic acids but apart from the nuclear envelope the role of the nuclear lipids in the functional organization of the cell nucleus was less studied. Nevertheless, nuclear lipids and specifically phosphatidylinositol phosphates (PIPs) play increasingly evident roles in gene expression. Therefore, here we provide the SMLM-based approach for the quantitative evaluation of the nuclear PIPs distribution while preserving the context of nuclear architecture. | |
| 2021 | |
| Artículo | |
| MethodsX, 8, 101372, 2021. | |
| Inglés | |
| Hoboth, P., Šebesta, O., Sztacho, M., Castano, E., & Hozák, P. (2021). Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates. MethodsX, 8, 101372. | |
| BIOLOGÍA MOLECULAR DE PLANTAS | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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| 2021_Peter_Hoboth.pdf | 4.45 MB | Adobe PDF | Visualizar/Abrir |