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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2773| Effect of PEG grafting density on surface properties of polyurethane substrata and the viability of osteoblast and fibroblast cells | |
| ANTONIO DAVID ABREU REJON WILBERTH ANTONIO HERRERA KAO ALEJANDRO MAY PAT ALEJANDRO AVILA ORTEGA NAYELI RODRIGUEZ FUENTES Jorge Alonso Uribe Calderón JOSE MANUEL CERVANTES UC | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.1007/s10856-022-06668-1 | |
| FIBROBLASTS OSTEOBLASTS PLANT EXTRACTS POLYETHYLENE GLYCOLS POLYURETHANES SURFACE PROPERTIES | |
| The surface of Tecoflex SG-80A Polyurethane (PU) films was modified by grafting polyethylene glycol (PEG) chains at three different molar amounts (0.05, 0.10, and 0.15 mmol). The resulting substrata were characterized by FTIR-ATR, TGA, AFM, SEM and contact angle to assess the surface modifications occurred during the grafting reactions. Osteoblasts and fibroblasts were cultured with PU extracts for 24 h, and their cell viability and morphology were evaluated by CellTiterBlue assay, Crystal Violet staining and Live/Dead assay. FTIR and TGA results indicated that PEG chains were successfully grafted onto PU surfaces, specifically in the hard segment of PU forming allophanate groups as the PEG grafting density increased. SEM and AFM images suggest that PU substrata were partially covered by PEG, increasing the dispersive and basic components of the PU surface energy. It was found that extracts from PEG-grafted polyurethanes increased the osteoblast viability, although fibroblasts viability remained constant regardless PEG grafting density; in spite of this both cells presented a more spread morphology at the lower PEG grafting density. Our results showed that surface energy of PU substrata can be tuned by PEG grafting density; also, the PEG leached tends to increase the pH of culture medium which leads to a higher viability of osteoblasts; nevertheless, PEG grafting density should be optimized to promote a healthy cell morphology as alterations in its morphology were detected at higher concentrations. [Figure not available: see fulltext.] © 2022, The Author(s). | |
| 2022 | |
| Artículo | |
| J Mater Sci: Mater Med 33, 45, 2022. | |
| Español | |
| Abreu-Rejón, A.D., Herrera-Kao, W., May-Pat, A. et al. Effect of PEG grafting density on surface properties of polyurethane substrata and the viability of osteoblast and fibroblast cells. J Mater Sci: Mater Med 33, 45 (2022). https://doi.org/10.1007/s10856-022-06668-1 | |
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| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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