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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/2813| Identification of a novel lipase with AHSMG pentapeptide in hypocreales and glomerellales filamentous fungi | |
| Denise Esther Gutiérrez Domínguez Bartolomé Humberto Chí Manzanero MARIA MERCEDES RODRIGUEZ ARGUELLO JEWEL NICOLE ANNA TODD Ignacio Rodrigo Islas Flores Miguel Canseco Blondy Beatriz Canto Canché | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.3390/ijms23169367 | |
| https://doi.org/10.3390/ijms23169367 | |
| EXTREME ENZYMES THERMOTOLERANT AND SOLVENT RESISTANT LIPASES AHSMG PENTAPEPTIDE PATHOGENICITY FACTORS NON-CANONICAL EFFECTORS FILAMENTOUS FUNGI | |
| Lipases are enzymes that hydrolyze triglycerides to fatty acids and glycerol. A typical element in lipases is a conserved motif of five amino acids (the pentapeptide), most commonly G-X-S-X-G. Lipases with the pentapeptide A-X-S-X-G are present in species of Bacillus, Paucimonas lemoignei, and the yeast Trichosporon asahii; they are usually thermotolerant and solvent resistant. Recently, while searching for true lipases in the Trichoderma harzianum genome, one lipase containing the pentapeptide AHSMG was identified. In this study, we cloned from T. harzianum strain B13-1 the lipase ID135964, renamed here as ThaL, which is 97.65% identical with the reference. We found that ThaL is a lid-containing true lipase of cluster III that belongs to a large family comprising highly conserved proteins in filamentous fungi in the orders Hypocreales and Glomerellales, in which predominantly pathogenic fungi are found. ThaL was expressed in conidia, as well as in T. harzianum mycelium, where it was cultured in liquid minimal medium. These results—together with the amino acid composition, absence of a signal peptide, mitochondrial sorting prediction, disordered regions in the protein, and lineage-specific phylogenetic distribution of its homologs—suggest that ThaL is a non-canonical effector. In summary, AHSMG-lipase is a novel lipase family in filamentous fungi, and is probably involved in pathogenicity. © 2022 by the authors. | |
| 2022 | |
| Artículo | |
| International Journal of Molecular Sciences, 23(16), 9367, 2022. | |
| Inglés | |
| "Gutiérrez-Domínguez, D.E.; Chí-Manzanero, B.; RodríguezArgüello, M.M.; Todd, J.N.A.; Islas-Flores, I.; Canseco-Pérez, M.Á.; Canto-Canché, B. Identification of a Novel Lipase with AHSMG Pentapeptide in Hypocreales and Glomerellales Filamentous Fungi. Int. J. Mol. Sci. 2022, 23, 9367.https://doi.org/10.3390/ijms23169367 | |
| BIOLOGÍA MOLECULAR | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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