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Detección UV-VIS de marcadores de diferenciación sexual en extractos hidroetanólicos de Vallisneria americana
Uv-vis detection of sexual differentiation markers in hydroethanolic extracts of Vallisneria americana
MARIA CONCEPCION JIMENEZ PEREZ
JESUS BAUTISTA REGIL
GUADALUPE HERNANDEZ PIEDRA
MARIA MARCELA GAMBOA ANGULO
ALBERTO DE JESUS SANCHEZ MARTINEZ
VIOLETA RUIZ CARRERA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.20937/RICA.54244
ANGIOSPERMA DIOICA
RESTAURACIÓN
PIGMENTOS SEXUALES
ESPECTROFOTOMETRÍA UV-VIS
The sexuality of submerged dioecious macrophytes acquires importance to strengthen research on environmental stress and repopulation of aquatic ecosystems. The objective of this work was to determine biomarkers that allow the sexual identification of Vallisneria americana by spectrophotometry analysis of the hydroethanolic extracts of its leaves or roots. Within the water column of the male and female vegetation, the parameters of pH, temperature, dissolved oxygen, conductivity, dissolved solids, and redox potential were measured in situ. For each reproductive morphotype, the number of leaves and the length of the predominant leaf and main root in each plant were analyzed. Spectral scanning of the extracts in the range of 340 to 750 nm was used both to compare the dominant wavelengths of the metabolites present and to quantify chlorophylls (a and b), carotenoids, and monomeric anthocyanins. The chlorophyll quotient was also obtained. The biomarkers of sexuality of this submerged macrophyte can be related to the content of the four pigments in the leaves that were invariably high in males, whereas the physics and chemistry of water and the vegetative morphology of each sex were almost similar. A possible microalgal influence is described by the inverse relationship of chlorophylls in roots and the finding of phycobilins in male leaves. The results offer the potential for ecological and biotechnological applications of V. americana aimed at the repopulation of wetlands affected by abiotic and biotic factors. © 2022, Centro de Ciencias de la Atmosfera, UNAM. All rights reserved.
2022
Artículo
Revista Internacional de Contaminación Ambiental, 38, 11-21, 2022.
Español
Jiménez-Pérez, M. C., Bautista-Regil, J., Hernández-Piedra, G., Gamboa-Angulo, M., de Jesús Sánchez, A., & Carrera, V. R. (2022). Detección UV-VIS de marcadores de diferenciación sexual en extractos hidroetanólicos de Vallisneria americana. Revista Internacional de Contaminación Ambiental, 38, 11-21.
BIOLOGÍA MOLECULAR
Versión publicada
publishedVersion - Versión publicada
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