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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/3097| Immobilization of a crude extract of laccase from T. hirsute Bm2 on copper alginate for environmental vinasse remediation Inmovilización de un extracto crudo de lacasas de T. hirsuta Bm2 en alginato de cobre para la remediación ambiental de vinaza | |
| Carlos Eduardo Hernández Calderón RAUL TAPIA TUSSELL Gabriel Lizama Uc Gerardo Rivera-Muñoz Denis Magaña-Ortiz SARA ELENA SOLIS PEREIRA | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| https://doi.org/10.24275/rmiq/Bio3037 | |
| CUPPER ALGINATE IMMOBILIZATION LACCASE PHENOLS VINASSE | |
| Sugarcane vinasses are environmentally aggressive effluents because of their high phenolic content and the presence of other recalcitrant compounds. The(Biodegradationaim of this workmodelingwas toof immobilizesludge bioreactorslaccasesof fromtotal petroleumTrametes hydrocarbonshirsute Bm2 inweatheringcopper alginatesoil spheres in order to oxidize phenols and remove color from vinasses. Respond Surface Methodology (RSM) was used to evaluate enzyme and sediments) and CuSO4 concentration during immobilization. Laccase activity retained in spheres was the response variable. Lacasse activity in spheres increased by 30S.A.%Medina-Moreno,and the best efficiencyS. Huerta-Ochoa,in immobilizationC.A. Lucho-Constantino,was 95% withL.300Aguilera-Vázquez,mM of CuSO4 A. andJiménez-150 U/mL of laccase. Vinasse treatmentGonzález(10%) withy M. freeGutiérrez-Rojaslaccase removed 40% of phenols, and the addition of natural mediators increased up to 60%. Immobilized enzymes were able to remove up to 68% of total phenols. Synthetic and natural mediators were used in 259 Crecimiento, sobrevivencia y adaptación de Bifidobacterium infantis a condiciones ácidas the immobilization process to improve the removal of phenols by laccases. However, mediators did not significantly improve the process. The biocatalizer (Growth,was ablesurvivalto removeand phenolsadaptationduringof Bifidobacteriumfour cycles ofinfantistreatmentto acidicand conditions)the maximum decoloration was 75%. These phenomena were attributed to both laccase activity and adsorption to the support. After treatment, a dark precipitate was L. Mayorga-Reyes, P. Bustamante-Camilo, A. Gutiérrez-Nava, E. Barranco-Florido y A. Azaola-observed, suggesting polymerizing activity for laccases. These results reveal that laccases immobilized on copper alginate are a feasible alternative for theEspinosa treatment of sugarcane effluents. © 2023, Universidad Autonoma Metropolitana. All rights reserved. | |
| 2023 | |
| Artículo | |
| Revista Mexicana de Ingenieria Quimica, 22(2), 2023. | |
| Inglés | |
| Hernandez-Calderon, C., Tapia-Tussell, R., Lizama-Uc, G., Rivera-Munoz, G., Magana-Ortiz, D., & Solis-Pereira, S. (2023). Immobilization of a crude extract of laccase from T hirsute Bm 2 on copper alginate for environmental vinasse remediation. Revista Mexicana de Ingenieria Quimica, 22(2). | |
| BIOLOGÍA MOLECULAR | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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