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Theoretical study of ArcB and its dimerization, interaction with anaerobic metabolites, and activation of ArcA
LUIS FELIPE PADILLA VACA
FRANCISCO JAVIER DE LA MORA BRAVO
RODOLFO GARCIA CONTRERAS
Jorge Humberto Ramírez Prado
MARCOS VICENTE GOMEZ
FRANCISCO VARGAS GASCA
LUIS FERNANDO ANAYA VELAZQUEZ
ITZEL PARAMO PEREZ
ANGELES RANGEL SERRANO
Hortensia Patricia Cuéllar Mata
NAURU IDALIA VARGAS MAYA
BERNARDO FRANCO BARCENAS
Acceso Abierto
Atribución-NoComercial-SinDerivadas
10.7717/peerj.16309
ArcB
TWO-COMPONENT SYSTEMS
PROTEIN MODELING
ALPHAFOLD2
SIGNALING MECHANISM
KINASE REGULATION
The complex metabolism of Escherichia coli has been extensively studied, including its response to oxygen availability. The ArcA/B two-component system (TCS) is the key regulator for the transition between these two environmental conditions and has been thoroughly characterized using genetic and biochemical approaches. Still, to date, limited structural data is available. The breakthrough provided by AlphaFold2 in 2021 has brought a reliable tool to the scientific community for assessing the structural features of complex proteins. In this report, we analyzed the structural aspects of the ArcA/B TCS using AlphaFold2 models. The models are consistent with the experimentally determined structures of ArcB kinase. The predicted structure of the dimeric form of ArcB is consistent with the extensive genetic and biochemical data available regarding mechanistic signal perception and regulation. The predicted interaction of the dimeric form of ArcB with its cognate response regulator (ArcA) is also consistent with both the forward and reverse phosphotransfer mechanisms. The ArcB model was used to detect putative binding cavities to anaerobic metabolites, encouraging testing of these predictions experimentally. Finally, the highly accurate models of other ArcB homologs suggest that different experimental approaches are needed to determine signal perception in kinases lacking the PAS domain. Overall, ArcB is a kinase with features that need further testing, especially in determining its crystal structure under different conditions. Copyright 2023 Padilla-Vaca et al.
2023
Artículo
PeerJ 11:e16309 DOI 10.7717/peerj.16309
Inglés
Padilla-Vaca F, de la Mora J, García-Contreras R, Ramírez-Prado JH, Vicente-Gómez M, Vargas-Gasca F, AnayaVelázquez F, Páramo-Pérez I, Rangel-Serrano Á, Cuéllar-Mata P, Vargas-Maya NI, Franco B. 2023. Theoretical study of ArcB and its dimerization, interaction with anaerobic metabolites, and activation of ArcA. PeerJ 11:e16309 DOI 10.7717/peerj.16309
BIOLOGÍA MOLECULAR DE PLANTAS
Versión publicada
publishedVersion - Versión publicada
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