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Theoretical study of ArcB and its dimerization, interaction with anaerobic metabolites, and activation of ArcA | |
LUIS FELIPE PADILLA VACA FRANCISCO JAVIER DE LA MORA BRAVO RODOLFO GARCIA CONTRERAS Jorge Humberto Ramírez Prado MARCOS VICENTE GOMEZ FRANCISCO VARGAS GASCA LUIS FERNANDO ANAYA VELAZQUEZ ITZEL PARAMO PEREZ ANGELES RANGEL SERRANO Hortensia Patricia Cuéllar Mata NAURU IDALIA VARGAS MAYA BERNARDO FRANCO BARCENAS | |
Acceso Abierto | |
Atribución-NoComercial-SinDerivadas | |
10.7717/peerj.16309 | |
ArcB TWO-COMPONENT SYSTEMS PROTEIN MODELING ALPHAFOLD2 SIGNALING MECHANISM KINASE REGULATION | |
The complex metabolism of Escherichia coli has been extensively studied, including its response to oxygen availability. The ArcA/B two-component system (TCS) is the key regulator for the transition between these two environmental conditions and has been thoroughly characterized using genetic and biochemical approaches. Still, to date, limited structural data is available. The breakthrough provided by AlphaFold2 in 2021 has brought a reliable tool to the scientific community for assessing the structural features of complex proteins. In this report, we analyzed the structural aspects of the ArcA/B TCS using AlphaFold2 models. The models are consistent with the experimentally determined structures of ArcB kinase. The predicted structure of the dimeric form of ArcB is consistent with the extensive genetic and biochemical data available regarding mechanistic signal perception and regulation. The predicted interaction of the dimeric form of ArcB with its cognate response regulator (ArcA) is also consistent with both the forward and reverse phosphotransfer mechanisms. The ArcB model was used to detect putative binding cavities to anaerobic metabolites, encouraging testing of these predictions experimentally. Finally, the highly accurate models of other ArcB homologs suggest that different experimental approaches are needed to determine signal perception in kinases lacking the PAS domain. Overall, ArcB is a kinase with features that need further testing, especially in determining its crystal structure under different conditions. Copyright 2023 Padilla-Vaca et al. | |
2023 | |
Artículo | |
PeerJ 11:e16309 DOI 10.7717/peerj.16309 | |
Inglés | |
Padilla-Vaca F, de la Mora J, García-Contreras R, Ramírez-Prado JH, Vicente-Gómez M, Vargas-Gasca F, AnayaVelázquez F, Páramo-Pérez I, Rangel-Serrano Á, Cuéllar-Mata P, Vargas-Maya NI, Franco B. 2023. Theoretical study of ArcB and its dimerization, interaction with anaerobic metabolites, and activation of ArcA. PeerJ 11:e16309 DOI 10.7717/peerj.16309 | |
BIOLOGÍA MOLECULAR DE PLANTAS | |
Versión publicada | |
publishedVersion - Versión publicada | |
Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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