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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/33| Annotation of Differentially Expressed Genes in the Somatic Embryogenesis of Musa and Their Location in the Banana Genome | |
| ROSA MARIA ESCOBEDO GRACIA MEDRANO | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Somatic embryogenesis is a powerful tool for the massive production of elite plant materials, as well as for molecular agricultural breeding through the use of biotechnological strategies. Although this technology can be applied to any plant species, it is particularly valuable for the asexually propagated ones, such as bananas (Musa spp.). Bananas are one of the most consumed fruits worldwide and represent an important source of revenue for tropical countries where they also account for one of the main staple foods. In spite of their nutritional and socioeconomic importance, molecular tools for genetic improvement of bananas are limited in comparison to other plant species. In addition, the molecular bases of zygotic and somatic embryogenesis in Musa are not fully understood. Recently, the genomic sequence of the double haploid banana-Pahang CIRAD 930 ITC 1511 (hereafter ITC 1511) was recently released [1]. ITC 1511 is derived from the Pahang wild diploid (2𝑛 = 22) Musa acuminata Colla. ssp. malaccensis accession which shares its genetic lineage with dessert and cooking bananas. The DH-Pahang genome size is 523 Mb (1C estimated through flow cytometry), and in a 91% assemblage it revealed 36,542 protein-coding genes anchored to the 11 Musa chromosomes. This provides a unique platform for genetic improvement of this underresearched vital crop. Besides the protein-coding genes, those for 235 microRNAs (MIR), corresponding to 37 different families, were found, including the eight families typical of Poaceae [1]. Using M. acuminata ssp. malaccensis immature zygotic embryos (IZE) we have developed an efficient somatic embryogenesis protocol, based on modifications to the one previously reported [2]. Moreover, we have observed changes in gene expression patterns during this process. Here, taking advantage of the information made available 2 The Scientific World Journal regarding the Musa genome, we were able to assign putative functions to some of these genes as well as to localize their position into the chromosomes of the Musa genome available. Our finding suggest that the cDNA-AFLP procedure was useful for identifying expressed genes during early and late zygotic and somatic embryogenesis in M. acuminata ssp. malaccensis and helping annotate them in the Musa genome, such as cytidine triphosphate synthase 2 (CTP synthase 2), serine/threonine protein kinase, starch branching enzyme (SBE1), early responsive to dehydrat | |
| 22-08-2013 | |
| Artículo | |
| BIOLOGÍA Y QUÍMICA | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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| 29061.pdf | 2 MB | Adobe PDF | Visualizar/Abrir |