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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/42| A SELECTIVE CHEMICAL METHOD FOR THE SEPARATION OF QUINONES FROM THE STEM BARK OF DIOSPYROS ANISANDRA | |
| ROCIO DE LOURDES BORGES ARGAEZ | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| Stem bark extract from Diospyros anisandra has been shown to possess significant antimycobacterial activity against a resistant strain of M. tuberculosis. This activity was detected in the quinone-rich fractions of the non polar extract. The bioassay-guided fractionation of the non-polar extract, resulted in a considerable amount of known terpenoids, and fatty esters, making it difficult to isolate the active quinoid components. This article deals with the comparison of four methods of extraction in combination with KOH/HCl treatment for the separation of slightly polar quinones from the terpenoids and fatty ester mixture. Ground plant material was extracted with n-hexane by static maceration (24 h), dynamic maceration (24 h), Soxhlet extraction (4 h) and ultrasonic extraction (2 h). The extraction efficiencies were measured and then, each extract was dissolved in chloroform and partitioned with an aqueous solution of KOH 5%; the aqueous solution was washed with chloroform, acidified until pH 5 with a chloride acid solution and extracted again with chloroform. The comparison of each fraction obtained was carried out by TLC and CG-MS analysis. Quantification of each quinone component by GC-MS revealed that plumbagin and droserone were the major naphthoquinones in the hexanic extract of the steam bark of this species. The four methods employed in combination with KOH/HCl allowed the separation of slightly polar quinones from terpenoids and fatty ester mixtures from the stem bark extract of D.anisandra | |
| 29-11-2013 | |
| Artículo | |
| CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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