Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/552
Antibacterial activity of defensin PaDef from Avocado Fruit (Persea americana var. drymifolia) Expressed in endothelial cells against Escherichia coli and Staphylococcus aureus
JAQUELINA JULIA GUZMAN RODRIGUEZ
RODOLFO LOPEZ GOMEZ
RAFAEL SALGADO GARCIGLIA
LUIS CARLOS RODRIGUEZ ZAPATA
ALEJANDRA OCHOA ZARZOSA
JOEL EDMUNDO LOPEZ MEZA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: 10.1155/2013/986273
COMPLEMENTARY DNA
DEFENSIN
DEFENSIN PADEF
ELONGATION FACTOR 1ALPHA
POLYPEPTIDE ANTIBIOTIC AGENT
UNCLASSIFIED DRUG
Antimicrobial therapy is a useful tool to control infectious diseases in general and rising antibiotic resistant microorganisms in particular. Alternative strategies are desirable, and antimicrobial peptides (AMP) represent attractive control agents. Mexican avocado (Persea americana var. drymifolia) is used in traditional medicine; however, the AMP production has not been reported in this plant. We obtained a cDNA library from avocado fruit and clone PaDef was identified, which has a cDNA (249 bp) encoding a protein (78 aa) homologous with plant defensins (>80%). We expressed the defensin PaDef cDNA (pBME3) in the bovine endothelial cell line BVE-E6E7. Polyclonal and clonal populations were obtained and their activity was evaluated against Escherichia coli, Staphylococcus aureus, and Candida albicans. E. coli viability was inhibited with 100 g/mL of total protein from clones (>55%). Also, S. aureus viability was inhibited from 50 g/mL total protein (27-38%) but was more evident at 100 g/mL (52-65%). This inhibition was higher than the effect showed by polyclonal population (23%). Finally, we did not detect activity against C. albicans. These results are the first report that shows antimicrobial activity of a defensin produced by avocado and suggest that this AMP could be used in the control of pathogens.
2013
Artículo
BioMed Research International, 2013 doi:10.1155/2013/986273
Inglés
Guzmán-Rodríguez, J. J., López-Gómez, R., Suárez-Rodríguez, L. M., Salgado-Garciglia, R., Rodríguez-Zapata, L. C., Ochoa-Zarzosa, A., & López-Meza, J. E. (2013). Antibacterial activity of defensin PaDef from avocado fruit (persea americana var. drymifolia) expressed in endothelial cells against escherichia coli and staphylococcus aureus. BioMed Research International, 2013 doi:10.1155/2013/986273
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

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