Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/776
Characterization of a Capsicum chinense seed peptide fraction with broad antibacterial activity
LIGIA GUADALUPE BRITO ARGAEZ
FERNANDO FEDER MOGUEL SALAZAR
FERNANDO ZAMUDIO ZUÑIGA
TOMAS GONZALEZ ESTRADA
IGNACIO RODRIGO ISLAS FLORES
Acceso Abierto
Atribución-NoComercial-SinDerivadas
 DOI: 10.3923/ajb.2009.77.87
ANTIMICROBIAL PEPTIDES
CAPSICUM CHINENSE
COLUMN CHROMATOGRAPHY
HABANERO PEPPER
PLANT ANTIMICROBIAL PEPTIDES
Habanero chili pepper (Capsicum chinense) is widely consumed as a fresh vegetable, although its extremely high capsaicin content has led to other uses (e.g., medicine and self-defense). Recently described antimicrobial peptides from C. annuum were very efficient in inhibiting growth in human and plant pathogenic bacteria and fungi. In order to explore the potential use of Capsicum chinenseseeds as a source of antimicrobial peptides, in the present study a peptide fraction from C. chinense pepper seeds, denominated G10P1, was enriched, partially purified and its antimicrobial activitytested against the plant and human pathogens Xanthomonas campestris, Pseudomonas syringae, Pseudomonas aeruginosa, Erwinia carotovora, Agrobacterium sp., Shigella flexnerii, Escherichia coli, Staphyllococcus aureus and Bacillus subtilis. The minimum inhibitory concentration of the G10P1 peptide fraction against X. campestris was 12.5 μg mL-1. Electrophoresis of the G10P1 in a denaturant 15% polyacrylamide gel showed it to be composed of ~7.57 and ~5.6 kDa polypeptides, both associated with an area of strong antibacterial activity. The sequencing of 18 amino acids from the N-terminal of the ~7.57 peptides and 12 from the ~5.6 kDa peptides showed no clear association with previously described antimicrobial peptides. However, the ~5.6 kDa peptides were related to the NAC and WRKY transcription factors, both involved in direct regulation of the plant defense response against pathogen attack and the ~7.57 kDa peptides had low homology with a 3-oxo-[acyl-carrier-protein] synthase from Capsicum chinense.
2009
Artículo
Asian Journal of Biochemistry, 4(3), 77-87. doi:10.3923/ajb.2009.77.87
Inglés
Brito-Argaez, L., Moguel-Salazar, F., Zamudio, F., Gonzalez-Estrada, T., & Islas-Flores, I. (2009). Characterization of a capsicum chinense seed peptide fraction with broad antibacterial activity. Asian Journal of Biochemistry, 4(3), 77-87. doi:10.3923/ajb.2009.77.87
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

Upload archives


File Description SizeFormat 
2009_AI_id18085_IslasFlores-BritoL.pdf1.79 MBAdobe PDFView/Open