Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/782
Encapsulación de embriones somáticos de Laelia anceps ssp. dawsonii para la producción de semilla sintética
Somatic embryoids encapsulation of Laelia anceps ssp. dawsonii to produce synthetic seed
HILDA EULALIA LEE ESPINOSA
JOAQUIN MURGUIA GONZALEZ
ANTONIO LAGUNA CERDA
MARTIN ROBERTO GAMEZ PASTRANA
MARIA ELENA GALINDO TOVAR
LOURDES GEORGINA IGLESIAS ANDREU
NANCY SANTANA BUZZY
Acceso Abierto
Atribución-NoComercial-SinDerivadas
ARTIFICIAL SEED
ORCHIDACEAE
SOMATIC EMBRYOGENESIS
Somatic embryoids obtained from germinated seeds in an MS culture media supplemented with naphthalene acetic acid (NAA), 6, bencylaminopurine (BAP) and indole-3-acetic acid (IAA), 2 mg·liter-1each, were encapsulated in sodium alginate in complex with calcium chloride (CaCl 2·2H2O) 75 mM matrix, to produce synthetic seeds, as a rescue and conservation strategy. In order to determinate the effect of physical and chemical factors, such as stored temperature and time, sodium alginate consistency, and mineral nutriments an plant growth regulators concentration on percentage and time for germination, and the viability of the synthetic seeds, different concentrations of: sodium alginate (2.0, 3.0 and 4.0 %), MS salts (37.5, 50 and 100 %) and BAP (0.5, 1.0 and 2.0 mg·liter -1) added to the encapsulation matrix, were tested at different temperatures (4, 20 and 25 °C) and incubation times (15 and 30 days). A hundred percent germination of synthetic seeds was obtained when 3 % sodium alginate in complex with CaCl2·2H2O at 75 mM was used; MS salts at 100 and 50 % and 2.0 mg·liter-1 BAP supplemented to the encapsulation matrix, achieved 64.54 y 62.77 % germination rate, respectively. No meaningful difference was observed between concentrations.
2009
Artículo
Revista Chapingo, Serie Horticultura, 15(2 SPL), 33-40, 2009
Inglés
Lee-Espinosa, H. E., Murguía-González, J., Laguna-Cerda, A., García-Rosas, B., Gámez-Pastrana, M. R., Galindo-Tovar, M. E., . . . Santana-Buzzy, N. (2009). Somatic embryoids encapsulation of laelia anceps ssp. dawsonii to produce synthetic seed. [Encapsulación de embriones somáticos de Laelia anceps ssp. dawsonii para la producción de semilla sintética] Revista Chapingo, Serie Horticultura, 15(2 SPL), 33-40.
BIOLOGÍA Y QUÍMICA
Versión publicada
publishedVersion - Versión publicada
Appears in Collections:Artículos de Investigación Arbitrados

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