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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/797| Isolation and Characterization of a Soluble Phosphate Hydrolysing Activity from an in vitro Coffee Cell Line Grown in the Presence of Aluminum | |
| YERENI MINERO GARCÍA MARCO ANTONIO VILLANUEVA MENDEZ FELIPE AUGUSTO VAZQUEZ FLOTA SOLEDAD MARIA TERESA HERNANDEZ SOTOMAYOR IGNACIO RODRIGO ISLAS FLORES | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| IN VITRO COFFEE CELL CULTURES PHOSPHATE HYDROLYSING ACTIVITY PLANT PROTEIN PURIFICATION ALLLLILIMUN STRESS | |
| As our interest is focused in obtaining a betler knowledge of the plant phosphate metabolism and its interactions with Al ion, we used an in vitro coffee cellline (Coffia arabica L.) tolerant to allllIlimun ion to purify a protein with phosphate hydrolysing activity with the airn to analyze whether the enzyrne can be contributing to the Al-tolerance in this coffee cellline. The protein was purified at least 138-fold; silver stain on a 10% SDS-PAGE detected a partially purified 30 kDa polypeptide which showed ability to hydrolyse phosphate either in native gels or soluble assays. The semipurified protein is able to hydrolyse sodiurn pyrophosphate (PPi-Na) and adenosine triphosphate (ATP) very efficiently, a1lhough P-serine (P-ser), P-Threonine (P-thr), P-Tyrosine (P-Tyr), Phytate, D-Myo-lnositol-lP (D-Myo Inos-lP) and lhe synlhetic p-nyliophenyl phosphate (P-NPP) could also be used as substrates. Enzyrnatic activity ofthe EDTA-inactivated enzyrne can be restored by Mg2+, as well as other divalent cations such as Fe2+, Co2+, Cu2+, Zn2+, Ca2+ and Mn2+. In contrast, Al3+ could only partially reactivate the phosphate hydrolysing activity, which suggests that it is not a cofactor for this enzyrne. Vv'hen Al3 + was added to the Mg2+ -enzyrne complex, it strongly inhibited the enzyrnatic activity either, exerting a negative effect over the enzyrne or the substrate. Between a munber of phosphohydrolase inhibitors, only KN03 was able to decrease the activity suggesting that this enzyme should be related with the V-ATPase protein family or with plant phosphatases. | |
| 2007 | |
| Artículo | |
| Asian Journal of Biochemistry. 2. 302-313. 10.3923/ajb.2007.302.313. | |
| Inglés | |
| Yereni, Minero-Garcia & Villanueva, Marco & Vazquez-Flota, Felipe & S.M. Teresa, Hernandez-Sotomayor & Islas-Flores, Ignacio. (2007). Isolation and Characterization of a Soluble Phosphate Hydrolysing Activity from an in vitro Coffee Cell Line Grown in the Presence of Aluminum. Asian Journal of Biochemistry. 2. 302-313. 10.3923/ajb.2007.302.313. | |
| BIOLOGÍA Y QUÍMICA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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