Please use this identifier to cite or link to this item: http://cicy.repositorioinstitucional.mx/jspui/handle/1003/9
A quantitative PCR approach for determining the ribosomal DNA copy number in the genome of Agave tequila Weber
LORENZO FELIPE SÁNCHEZ TEYER
Acceso Abierto
Atribución-NoComercial-SinDerivadas
http://dx.doi.org/10.1016/j.ejbt.2016.05.002
http://dx.doi.org/10.1016/j.ejbt.2016.05.002
Background: Agave tequilana has a great economic importance inMexico in order to produce alcoholic beverages and bioenergy. However, in this species the structure and organization of the rDNAs in the genome are limited, and it represents an obstacle both in their genetic research and improvement as well. rDNA copy number variations per eukaryotic genome have been considered as a source of genetic rearrangements. In this study, the copy number of 18S and 5S rDNAs in the A. tequilana genome was estimated, and an absolute quantitative qPCR assay and genome size was used. In addition, an association between the rDNAs copy number and physical mapping was performed to confirm our results. Results: The analysis were successfully applied to determine copy number of 18S and 5S rDNAs in A. tequilana genome, showing high reproducibility with coefficient of variation (CV) values of 0.014–0.0129%, respectively. A variation of 51 times in the copy number the 18s regarding 5s rDNA was found, thus contributing to genome size of 1.47 and 8.38 × 10-3%, respectively. Similarly, data show a linear relationship (R [2] = 0.992) between rDNA copy number and the detected signals for each of the loci by FISH. The comparison of the rDNA copy number of agave showed differential relationshipwith other organisms and it may be due to evolutionary ecology. Conclusions: Results show that the proposed method a) can correctly detect the rDNA copy number, b) could be used as species-specific markers and c) might help in understanding the genetic diversity, genome organization and evolution of this species.
02-06-2016
Artículo
Publicación electrónica
Inglés
Internacional
Investigadores
J. Rubio-Piña et al. / Electronic Journal of Biotechnology 22 (2016) 9–15
OTRAS
Appears in Collections:Artículos de Investigación Arbitrados

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