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http://cicy.repositorioinstitucional.mx/jspui/handle/1003/929| A fast, simple, and reliable high-yielding method for DNA extraction from different plant species | |
| RAUL TAPIA TUSSELL ANDRES FELIPE DE JESUS QUIJANO RAMAYO RAFAEL ANTONIO ROJAS HERRERA FRANCISCO ALFONSO LARQUE SAAVEDRA DAISY DE LA CARIDAD PEREZ BRITO | |
| Acceso Abierto | |
| Atribución-NoComercial-SinDerivadas | |
| DOI: 10.1385/MB:31:2:137 | |
| AGAVACEAE CARIACACEAE CUCURBITACEAE DNA ISOLATION GRAMINAEAE PALMAE | |
| Genetic studies and pathogen detection in plants using molecular methods require the isolation of DNA from a large number of samples in a short time span. A rapid and versatile protocol for extracting high-quality DNA from different plant species is described. This method yields from 1 to 2 mg of DNA per gram of tissue. The absorbance ratios (A260/A280) obtained ranged from 1.6 to 2.0. A minimal presence of contaminating metabolites (as polymerase chain reaction [PCR] inhibitors) in samples and a considerable savings in reagents are characteristics of this protocol, as well as the low cost of the analysis per sample. The quality of the DNA was suitable for PCR amplification. | |
| 2005 | |
| Artículo | |
| Molecular biotechnology, 31(2), 137-139, 2005 | |
| Inglés | |
| Tapia-Tussell, R., Quijano-Ramayo, A., Rojas-Herrera, R., Larque-Saavedra, A., & Perez-Brito, D. (2005). A fast, simple, and reliable high-yielding method for DNA extraction from different plant species. Molecular biotechnology, 31(2), 137-139. | |
| CIENCIAS DE LA VIDA | |
| Versión publicada | |
| publishedVersion - Versión publicada | |
| Aparece en las colecciones: | Artículos de Investigación Arbitrados |
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