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Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots
ILEANA DE LA CARIDAD ECHEVARRIA MACHADO
ANGELA FRANCISCA KU GONZALEZ
VICTOR MANUEL LOYOLA VARGAS
SOLEDAD MARIA TERESA HERNANDEZ SOTOMAYOR
Acceso Abierto
Atribución-NoComercial-SinDerivadas
https://doi.org/10.1111/j.0031-9317.2004.0212.x
BIOLOGICAL ORGANS
ENZYME INHIBITION
ENZYMES
IMMUNOLOGY
PHYSIOLOGY
PRECIPITATION (CHEMICAL) 
In Cantharanthus roseus transformed roots, the application of methylglyoxal bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.50), inhibited the root growth in a dose-dependent manner with a DL50 of about 300 μm. Spermidine and spermine (Spm) levels and SAMDC and phospholipase C (PLC; EC 3.1.4.3) activities were reduced in the presence of the inhibitor. The inhibition was reversed by the addition of Spm. Radioactivity from [14C[Spm was detected in an immunoprecipitated fraction with an antibody anti-PLC-δ. To our knowledge, this is the first direct evidence that demonstrates an interaction of Spm with the signal transduction cascade phosphoinositide-Ca 2+.
2004
Artículo
Physiologia plantarum, 120(1), 140-151, 2004
Español
Echevarría‐Machado, I., Ku‐González, A., Loyola‐Vargas, V. M., & Hernández‐Sotomayor, S. M. (2004). Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots. Physiologia plantarum, 120(1), 140-151.
CIENCIAS DE LA VIDA
Versión publicada
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