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Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots | |
ILEANA DE LA CARIDAD ECHEVARRIA MACHADO ANGELA FRANCISCA KU GONZALEZ VICTOR MANUEL LOYOLA VARGAS SOLEDAD MARIA TERESA HERNANDEZ SOTOMAYOR | |
Acceso Abierto | |
Atribución-NoComercial-SinDerivadas | |
https://doi.org/10.1111/j.0031-9317.2004.0212.x | |
BIOLOGICAL ORGANS ENZYME INHIBITION ENZYMES IMMUNOLOGY PHYSIOLOGY PRECIPITATION (CHEMICAL) | |
In Cantharanthus roseus transformed roots, the application of methylglyoxal bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC; EC 4.1.1.50), inhibited the root growth in a dose-dependent manner with a DL50 of about 300 μm. Spermidine and spermine (Spm) levels and SAMDC and phospholipase C (PLC; EC 3.1.4.3) activities were reduced in the presence of the inhibitor. The inhibition was reversed by the addition of Spm. Radioactivity from [14C[Spm was detected in an immunoprecipitated fraction with an antibody anti-PLC-δ. To our knowledge, this is the first direct evidence that demonstrates an interaction of Spm with the signal transduction cascade phosphoinositide-Ca 2+. | |
2004 | |
Artículo | |
Physiologia plantarum, 120(1), 140-151, 2004 | |
Español | |
Echevarría‐Machado, I., Ku‐González, A., Loyola‐Vargas, V. M., & Hernández‐Sotomayor, S. M. (2004). Interaction of spermine with a signal transduction pathway involving phospholipase C, during the growth of Catharanthus roseus transformed roots. Physiologia plantarum, 120(1), 140-151. | |
CIENCIAS DE LA VIDA | |
Versión publicada | |
publishedVersion - Versión publicada | |
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