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Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy
CECILIA GUIZAR GONZALEZ
MIRIAM DEL SOCORRO MONFORTE GONZALEZ
FELIPE AUGUSTO VAZQUEZ FLOTA
Acceso Abierto
Atribución-NoComercial-SinDerivadas
DOI: 10.1007/s10529-016-2095-2
ARGEMONE MEXICANA
BENZOPHENANTHRIDINES
OCDA PATHWAY
SANGUINARINE
Exposure to YE promoted sanguinarine accumulation. This was not observed when they were exposed to methyl jasmonate (MeJa). Use of diethyldithiocarbamic acid (DIECA), an inhibitor of the OCDA pathway, resulted in partial impairment of this response. Exogenous application of MeJa did not reverse this effect in DIECA-exposed cultures. qRT-PCR revealed that the accumulation of transcripts corresponding to the berberine bridge enzyme gene, which was induced by YE exposure, was blocked by OCDA pathway and reversed by exogenous MeJa. Interestingly, this response pattern could not be observed on dihydrobenzophenanthridine oxidase enzyme activity, which was promoted by YE, but unaffected by either OCDA or MeJa.
2016
Artículo
Biotechnology letters, 38(7), 1237-1242, 2016.
Inglés
Guízar-González, C., Monforte-González, M., & Vázquez-Flota, F. (2016). Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy. Biotechnology letters, 38(7), 1237-1242.
CIENCIAS DE LA VIDA
Versión publicada
publishedVersion - Versión publicada
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